Trude Mostue faces a crisis when a disturbance causes her to make a potentially-fatal error in the operating theatre during a routine castration on a guinea pig named Pepper. In King's Lynn Craig Beck battles to save the life of a black and white farm cat named Boris, while his wife Alison tries to solve the mystery illness of a West Highland terrier.

Cheltenham vets Joe and Emma Inglis come back to earth with a bump after their honeymoon in the Dominican Republic. Joe is forced to amputate a fawn's leg if it is to survive an injury, while Emma faces an ethical question of how best to treat a family cat which has seen its best days. In Hull, Sam Robinson has to deal with a guinea pig's mystery pregnancy and then helps when a show pony has to undergo a tail amputation.

Keith Leonard is called out to help a collapsed cow, and Fiona Green treats an injured woodpecker. Meanwhile, Keith's elder brother Steve, who is now happily settled in Lancaster, treats a litter of orphaned hedgehogs ready for release back into the wild.

Trude Mostue fights to save the lives of a litter of kittens who have lost a large amount of blood. She tries heart massage, adrenaline injections and even mouth-to-mouth resuscitation. Later she tells her friend Maria Lowe about a new man in her life. Meanwhile, Alison Beck works against the clock operating on a cat with severe internal injuries following a road accident and her husband Craig treats a rabbit with an ulcer.

Keith Leonard is called out to perform a blood transfusion on a cow, and Trude Mostue takes care of Bonnie, a dog with a skin irritation. Meanwhile, Fiona Green attends to Clyde, a cat who was hit by a bus.

Being on call at night is an integral part of a vet's job, and Sam Robinson spends an evening struggling to save a horse with a potentially fatal condition, while Emma Inglis is called out to help Megan, an epileptic dog.

Craig and Alison Beck's celebrations of their first wedding anniversary are muted as Craig struggles to help Boss, a much-loved gun dog. Meanwhile, Emma Inglis turns inventor, and her husband Joe proves a reluctant father.

Trude Mostue thinks her new boyfriend Patrick Evans could be Mr. Right, but then has to worry about a Doberman's possible pregnancy. Hannah Pollard has a new look after shedding three stones, and then puts an overweight rabbit on a diet. Sam Robinson, who has little time to socialise, has to castrate a young stallion.

It's all change for Keith Leonard and Fiona Green. Keith is preparing to leave his job in Carmarthen but first, has to tackle his first hernia operation, on a calf. Fiona is off for a year on a working holiday as a diving instructor in the Caribbean but before that has to fit contact lenses on a King Charles spaniel.