Officer John Nolan and a sleep-deprived Celina investigate a cash deposit linked to an old DEA case. Meanwhile, Wesley grows increasingly concerned about his deposition and is shocked when he realizes Elijah’s attorney is a familiar face from his past.

Officer John Nolan and the team investigate the unexpected death of a suspect in police custody. Their search also uncovers a very delicate loose end that could jeopardize Officer Celina Juarez's career. Meanwhile, Sergeant Grey and his wife, Luna, go to New York to visit their daughter, Dominique, only to realize she never made it home from the night before.

Nolan and Celina must stand guard at a hospital after a dangerous prisoner needs surgery. Aaron, Lopez and Harper look into a string of robberies. Tim and Lucy consider how their secret relationship will affect their work. Lopez gets unexpected news.

With help from the FBI, Detective Lopez puts everything on the line to help Elijah Stone take down a gang leader in exchange for her safety. Meanwhile, Nolan and Bailey must deal with the secrets and shady dealings his late mother left behind.

When Dim goes missing, Officer Chen and Sergeant Bradford, along with the CIA, set out to find him and enlist the help of Juicy.

Officer Lucy Chen goes undercover to infiltrate an illegal firearm operation, and Detective Harper and Officer John Nolan work together to solve a mystery involving severed limbs found around the city.

The crossover continues on The Rookie: Feds S01E21 Bloodline (II).